| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256271 |
| Chromosome: | chromosome 8 |
| Location: | 2352496 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370750 | (1 of 1) IPR021934 - Sox C-terminal transactivation domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGATGACCCGAGCCCGCGCCTTCACTG |
| Internal bar code: | GTGGTGCGGGGATTACGCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 8 |
| LEAP-Seq percent confirming: | 99.4118 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAGCTGTTCCCTGACCC |
| Suggested primer 2: | CTGTTGCTGCTGTTGCTGAT |