| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256276 |
| Chromosome: | chromosome 2 |
| Location: | 2721685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093650 | (1 of 1) 1.14.12.22 - Carbazole 1,9a-dioxygenase / CARDO | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGGCACGAGGTCGCCGTGCCAGACAA |
| Internal bar code: | GGAACGAATGGTTGGCGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 99.2647 |
| LEAP-Seq n confirming: | 270 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGAGAAGAAATTCCAACA |
| Suggested primer 2: | CTCGAGACATTTCTAGGCGG |