Insertion junction: LMJ.RY0402.256282_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256282
Chromosome:chromosome 2
Location:3473335
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095120 ANK18 Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACTGCCACAAGACTTGCTCAGATGGATA
Internal bar code:TGGGCTCAATACTCGCATCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:463
LEAP-Seq percent confirming:97.2222
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACACAGTGGGACCGTACAC
Suggested primer 2:ATGATACTGAAAAACGGCGG