Insertion junction: LMJ.RY0402.256291_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256291
Chromosome:chromosome 9
Location:3719975
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCATGTAATGTTCAGCACTCGCGCGCAG
Internal bar code:GGCGAACAGGTGTGTCGTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:58
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCGTGACATTCTGCTCT
Suggested primer 2:TCGTCTACACAGCTCATCCG