| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256352 |
| Chromosome: | chromosome 16 |
| Location: | 5732830 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678850 | (1 of 1) 2.3.3.10 - Hydroxymethylglutaryl-CoA synthase / Hydroxymethylglutaryl coenzyme alpha-condensing enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATAGGATTGTGTGTGTCTTTGTGATGT |
| Internal bar code: | GTCCGCGAAGAGGTCCAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.4236 |
| LEAP-Seq n confirming: | 345 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCCAAAAGGCAAGAATA |
| Suggested primer 2: | GCTCATCCAGGTCAAACCAT |