Insertion junction: LMJ.RY0402.256371_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.256371
Chromosome:chromosome 6
Location:1018058
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g256050 (1 of 19) 3.6.1.3 - Adenosinetriphosphatase / Triphosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAGGGAGGAAGACTCCACCCGTCATGA
Internal bar code:GCTTAGTGCTCATCGCGCGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:156
LEAP-Seq percent confirming:99.7107
LEAP-Seq n confirming:1034
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCTGCTGTTTTGAGGGC
Suggested primer 2:CTGGAACTCCACTTTCCCAA