| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256420 |
| Chromosome: | chromosome 7 |
| Location: | 6370123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357550 | (1 of 3) K06236 - collagen, type I/II/III/V/XI/XXIV/XXVII, alpha (COL1AS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATAACCCTGTGGGTGGCGCACCACCCAT |
| Internal bar code: | AGCTAACGGGTAAGAAGCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 94.4732 |
| LEAP-Seq n confirming: | 547 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCATGTCCAACGACTCC |
| Suggested primer 2: | AAAGTACCACACAGGGCCAC |