| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256422 |
| Chromosome: | chromosome_3 |
| Location: | 7976957 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g201900 | RSP1 | Radial spoke protein 1 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAATACGATACTGCAAACTCCAGCTTGCT |
| Internal bar code: | TGCCCTTGTGGGGATCCTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 90.3005 |
| LEAP-Seq n confirming: | 1322 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGCAGTGAAACTGTGAA |
| Suggested primer 2: | TACGGTATTGGTAGGAGGCG |