| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256443 |
| Chromosome: | chromosome 7 |
| Location: | 1734420 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325715 | (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCATTCCTCAGTATTTGCTCGTAGTA |
| Internal bar code: | AGGTCAACTTTTCTTTTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 99.4039 |
| LEAP-Seq n confirming: | 1334 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAACTCTCCGTGTCTGC |
| Suggested primer 2: | ATACGCCATCCCACATTCAT |