| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256444 |
| Chromosome: | chromosome 16 |
| Location: | 4657694 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687400 | (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCTCGATGGGCACCACACCCCCGTGGC |
| Internal bar code: | GGGGGCCTCTATTTCACTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.3363 |
| LEAP-Seq n confirming: | 4191 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCAGCCCATGAAACTGT |
| Suggested primer 2: | TGGCAGTGACGAAGCTTATG |