| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256466 |
| Chromosome: | chromosome 4 |
| Location: | 3062676 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225250 | (1 of 3) PF14494 - Domain of unknown function (DUF4436) (DUF4436) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATGTGTACATGAAGCGAGAAGTGACGC |
| Internal bar code: | TCACGCCTCCCCGACCGATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.7209 |
| LEAP-Seq n confirming: | 1429 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGGGTCCTGTTTAATGA |
| Suggested primer 2: | CTTGTGTGTGTGTGTGCTGC |