| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256503 |
| Chromosome: | chromosome_3 |
| Location: | 8064777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g201200 | FAP283 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAACACTCAGAGCTCCATGTCCGCGGCCC |
| Internal bar code: | ATCTCGGTAACCAATTCGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 99.0196 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTGAACCACCACAAAT |
| Suggested primer 2: | AGGTGCACGGACTTCTCACT |