Insertion junction: LMJ.RY0402.256520_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256520
Chromosome:chromosome 10
Location:3044395
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g441300 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor gene_edge/mRNA_edge/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCAAAGTTTGCGTCACAACTCCATAAAT
Internal bar code:TCTCCGCGTTTGACCTCGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:608
LEAP-Seq percent confirming:99.2874
LEAP-Seq n confirming:418
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGCCGCACTACTTTGA
Suggested primer 2:TAGTGAGCCCGCTATCTCGT