| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256531 |
| Chromosome: | chromosome 9 |
| Location: | 2769961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389089 | CNX5,NOFNIR1,NOFNiR,ARC1 | NO-forming nitrite reductase; (1 of 1) PTHR14237:SF30 - MITOCHONDRIAL AMIDOXIME-REDUCING COMPONENT 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATAAATGTTAAGACCGCCGATTGTGAG |
| Internal bar code: | AGAGCGTGAATTCGTGTTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCAAGAGCAGTGGTTT |
| Suggested primer 2: | GGAAAAACTCGCAGAACAGC |