| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256578 |
| Chromosome: | chromosome 3 |
| Location: | 5334140 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCACAGGAAACTACCATAACGCGTTTG |
| Internal bar code: | AAGGGTACTCCTCGCAGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 99.7669 |
| LEAP-Seq n confirming: | 428 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAGTGAGCAGCTAGAGA |
| Suggested primer 2: | AACGCACAAGAAGGGGTATG |