| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256581 |
| Chromosome: | chromosome 7 |
| Location: | 5118578 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347950 | (1 of 54) IPR001680//IPR017986 - WD40 repeat // WD40-repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACCAGCGTTTTCTCATAGCAAACGCC |
| Internal bar code: | CGGCGCTTTTTTCCGACCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 88.2597 |
| LEAP-Seq n confirming: | 639 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGTGGGA |
| Suggested primer 2: | CAAACGTATTGATTGGCGTG |