Insertion junction: LMJ.RY0402.256583_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256583
Chromosome:chromosome 6
Location:8690492
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g309650 (1 of 1) IPR003200//IPR006626//IPR011050 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like // Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAACCGCGCATTTCAGTCTGGAGGCAAGT
Internal bar code:GGAGTGTGCGATAAACTCGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:595
LEAP-Seq percent confirming:99.1585
LEAP-Seq n confirming:707
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTTGAGAAGAACAACGC
Suggested primer 2:AAGCGTCAAGGGCAAAGTAA