| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256584 |
| Chromosome: | chromosome 3 |
| Location: | 5453286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185300 | GAK1,GALK1 | (1 of 1) 2.7.1.46 - L-arabinokinase; Galactokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTCGTGGGCACTGATATTGTGCGGTTA |
| Internal bar code: | TATACTACAGCGCTAACGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGGTGTGGAGGTGTCT |
| Suggested primer 2: | CTCCACTCACTCCTTCTCGG |