| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256642 |
| Chromosome: | chromosome 1 |
| Location: | 4959082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAATGGGGAGTGGGGATTTAGCAGAGA |
| Internal bar code: | GTCGAGTGCTTCGCTTTCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 97.9218 |
| LEAP-Seq n confirming: | 801 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCTAGACAACCGGAAG |
| Suggested primer 2: | TGTCAACAGAGAGCGGAGTG |