| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256657 |
| Chromosome: | chromosome 1 |
| Location: | 4736460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032700 | GLK1 | (1 of 1) 2.7.1.2 - Glucokinase / Glucose kinase; Glucokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGCCCAAGCGACCACGGCACGTTGCAC |
| Internal bar code: | CGGGGGACTCCGGTCAGGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 99.1853 |
| LEAP-Seq n confirming: | 487 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCTAGAGCCCCTTCCTC |
| Suggested primer 2: | CCCAACACAAGAGATTGGCT |