| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256663 |
| Chromosome: | chromosome 3 |
| Location: | 2975362 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163550 | (1 of 2) 4.2.1.130 - D-lactate dehydratase / Glyoxylase III | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACATGCAAGCGGTTCTTGTGCGCACGG |
| Internal bar code: | GAAGGCCAGAGCCCAGGAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 76.1905 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATTACTGCTCAAGGCA |
| Suggested primer 2: | CTCACTTCCTCACCTCCTCG |