Insertion junction: LMJ.RY0402.256665_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256665
Chromosome:chromosome 2
Location:4156807
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g098200 CGL103,PRT1 (1 of 1) PF00569//PF13920 - Zinc finger, ZZ type (ZZ) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3); Conserved in the Green Lineage 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTTACAGATGCGGGGCTGCCCTGATGAT
Internal bar code:ACTGGACAGGCTCACAAAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:633
LEAP-Seq percent confirming:99.3961
LEAP-Seq n confirming:1975
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGACATGTTCGACCTGG
Suggested primer 2:CACCGTTCCCTGTAAGCAAT