| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256683 |
| Chromosome: | chromosome 2 |
| Location: | 2014609 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088500 | CPLD29 | (1 of 1) 5.3.3.1 - Steroid Delta-isomerase / Steroid isomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGATTGTAGCGAAGGGGGGGTGGTTAGC |
| Internal bar code: | CGCGGGATCGCAGCATATACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 99.4761 |
| LEAP-Seq n confirming: | 5886 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCATCTGTTTGCATT |
| Suggested primer 2: | TAACAAACCCAGTCCGAAGC |