| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256714 |
| Chromosome: | chromosome 9 |
| Location: | 4642933 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396661 | DUO3,DIV39 | (1 of 2) IPR000104//IPR001005//IPR009057 - Antifreeze protein, type I // SANT/Myb domain // Homeodomain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTGACCCATGAGGCCTTACCTGCAGGA |
| Internal bar code: | GAGTGGTAGAGGGCGACCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCTGACAGCAAAATGGG |
| Suggested primer 2: | GAAGATGAGGAAGAGGTGCG |