Insertion junction: LMJ.RY0402.256728_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.256728
Chromosome:chromosome 4
Location:493866
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCCTTCCGCTGCTCCGCCGGCGCCCACC
Internal bar code:TCCCATGAAGAGCAATAGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:87
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGCTGGGTTGTAGAGGGT
Suggested primer 2:ACACACACACACACACACGG