| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256738 |
| Chromosome: | chromosome 15 |
| Location: | 1039492 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638651 | OPR80,CLR3,CLR20,RAP15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACTCTGTAATGGGAATCGGGTGCAGGC |
| Internal bar code: | CATGCTCCCTGGGTCTTCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 98.4925 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGGTATATGGAGCACT |
| Suggested primer 2: | GCTGCTGCTGTCACTGCTAC |