| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256743 |
| Chromosome: | chromosome_6 |
| Location: | 6308902 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g291700 | RSP3 | Radial spoke protein 3 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGGATATTTCTACAGCACCCCGGGCCTTG |
| Internal bar code: | GAGGGTAGTCGCAATTGGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 98.7603 |
| LEAP-Seq n confirming: | 239 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATGCCAGACAGGTAGCC |
| Suggested primer 2: | AGGATTCACCTTGCGAGCTA |