| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256756 |
| Chromosome: | chromosome 10 |
| Location: | 3507075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445150 | EXN12 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCCGTCTGGACGCCGCGACAGGCCGCG |
| Internal bar code: | GGGGGTTTCCTACGACGGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.3039 |
| LEAP-Seq n confirming: | 428 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGCAAGGGATTACCGT |
| Suggested primer 2: | AGCCAACATATTGACCTCGG |