Insertion junction: LMJ.RY0402.256790_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256790
Chromosome:chromosome 11
Location:3441933
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g481050 (1 of 10) PF00505 - HMG (high mobility group) box (HMG_box); High mobility group protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGACTGATTGGCACCCCACTGCTGATTG
Internal bar code:CCTAGCACCAACAGGGGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:607
LEAP-Seq percent confirming:88.9952
LEAP-Seq n confirming:744
LEAP-Seq n nonconfirming:92
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GGACAGACACTCGACGGATT
Suggested primer 2:CCTCACCCACCCAACTTCTA