| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256806 |
| Chromosome: | chromosome 1 |
| Location: | 5599992 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039750 | PDF1B | (1 of 1) PTHR10458//PTHR10458:SF2 - PEPTIDE DEFORMYLASE // PEPTIDE DEFORMYLASE 1B, CHLOROPLASTIC/MITOCHONDRIAL; Peptide deformylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACTCTGGGGGTAACGTGCACTTCCACC |
| Internal bar code: | ACTATGCTTGTAGAAACGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGTAACTGCGGCGTTTG |
| Suggested primer 2: | GCAGACTTCCATGCTCCTTC |