| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256818 |
| Chromosome: | chromosome_14 |
| Location: | 781027 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g612950 | CYG17 | Adenylate/guanylate cyclase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGTTGGGTCTGCGGCGGGGGCGTCAGGG |
| Internal bar code: | ATGATAGCCTAGGTGCGGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 98.9583 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGGGTACCAGCAAGGAG |
| Suggested primer 2: | ACAACCTCAATCCCTGCAAC |