| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256820 |
| Chromosome: | chromosome 3 |
| Location: | 4354512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175100 | (1 of 1) IPR013750//IPR016035 - GHMP kinase, C-terminal domain // Acyl transferase/acyl hydrolase/lysophospholipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCGGCCTTTCAGATCGGGTTGAACT |
| Internal bar code: | ACGGACACTGTCGTTAGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.604 |
| LEAP-Seq n confirming: | 4024 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTACCGACCCTGTTACG |
| Suggested primer 2: | CCCTAATAACGTAACCGCGA |