| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256825 |
| Chromosome: | chromosome 17 |
| Location: | 118241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697000 | (1 of 2) PTHR23155:SF401 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCCCCTGCCTCACCGCTACATGTCCCC |
| Internal bar code: | ATCGTCCTACCGCCGTTAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 15 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 460 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCATGGTACTGGTACTG |
| Suggested primer 2: | ACTTCACCTGTATGACCGCC |