| Insertion cassette: | CIB1 | 
| Side of cassette: | 3' | 
| Strand: | + | 
| Strain: | LMJ.RY0402.256825 | 
| Chromosome: | chromosome 17 | 
| Location: | 118343 | 
| Confidence (%): | 95 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre17.g697000 | (1 of 2) PTHR23155:SF401 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGCCTGGAGTTGACTCTGCCTTCACT | 
| Internal bar code: | AAGTGGCAGACGCTATGAGATC | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 | 
| LEAP-Seq percent confirming: | 100.0 | 
| LEAP-Seq n confirming: | 4 | 
| LEAP-Seq n nonconfirming: | 0 | 
| LEAP-Seq n unique pos: | 1 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGCTAAAATGTTGGGGA | 
| Suggested primer 2: | ACTTCACCTGTATGACCGCC |