| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256833 |
| Chromosome: | chromosome 6 |
| Location: | 2600815 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269544 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTCCCGGCCGCTCTCGAGCCCTTGCCA |
| Internal bar code: | TCTAGGAAAAGACGGCCGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 96.5636 |
| LEAP-Seq n confirming: | 281 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGTTGTGCGTATTTCC |
| Suggested primer 2: | CCTTTTGCAGGCTGCTTTAC |