| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256838 |
| Chromosome: | chromosome_14 |
| Location: | 2343142 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g623800 | NAT6 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGCCACCTCCTGAACAACCGCACCCCGCA |
| Internal bar code: | ATCCGGATCGATCGGGTCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 95.4167 |
| LEAP-Seq n confirming: | 229 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTAACGAAGATACCGGGC |
| Suggested primer 2: | CGCAAACACATGCTGATCTT |