| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256848 |
| Chromosome: | chromosome 2 |
| Location: | 234232 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074758 | CYG8 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGTACACTCCCCGGCTGGGCTGAACCA |
| Internal bar code: | TTTACTATTGGCTAGGTCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 98.0813 |
| LEAP-Seq n confirming: | 869 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACCCCTACGCAACAAGG |
| Suggested primer 2: | TGTAGTGGCGATGGGTGTAA |