Insertion junction: LMJ.RY0402.256867_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256867
Chromosome:chromosome 12
Location:6808228
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g561550 CDS1 Mitochondrial half-size ABC transporter, membrane protein; (1 of 2) K05661 - ATP-binding cassette, subfamily B (MDR/TAP), member 6 (ABCB6) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGAACCAGCTGCGTCGTCACCCGACA
Internal bar code:GGCGGCTCTGTGTTTCGAGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:536
LEAP-Seq percent confirming:98.3363
LEAP-Seq n confirming:2778
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCGCTTGTACAGGATGGG
Suggested primer 2:GACATGCGCTCTCGTACAAA