| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256878 |
| Chromosome: | chromosome 17 |
| Location: | 3918293 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728400 | (1 of 2) PTHR19241:SF62 - PROTEIN SCARLET | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGACCATACGACGTACAGGCAACCATA |
| Internal bar code: | AATATGGCCCTGGCAAACGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 94.7405 |
| LEAP-Seq n confirming: | 2756 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATAAGCAGAGAAGGCGTG |
| Suggested primer 2: | CGGTGTGATGCCGTACATAG |