| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256888 |
| Chromosome: | chromosome 12 |
| Location: | 4647748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523050 | CCT11,GEX204 | (1 of 1) 2.7.1.150 - 1-phosphatidylinositol-3-phosphate 5-kinase / Type III PIP kinase; Putative T-complex protein 1 component | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGTCAAAACACCCAGTAGCCGCTATTC |
| Internal bar code: | GGTGGGTAGGGCTGTAGAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTGCTCGCTATCTCTTA |
| Suggested primer 2: | CCAACTTTGTTCGGTTTCGT |