| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256894 |
| Chromosome: | chromosome 3 |
| Location: | 3055740 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164350 | (1 of 1) K06130 - lysophospholipase II (LYPLA2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACATTGGCGCCCAGCGACTGCAGCAGC |
| Internal bar code: | CCTGACGACGATGTCGCGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 99.4877 |
| LEAP-Seq n confirming: | 2719 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCACCACTACCTCCACA |
| Suggested primer 2: | TACGCTCCACACCTCTTCCT |