| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256899 |
| Chromosome: | chromosome 1 |
| Location: | 562697 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003200 | (1 of 1) 1.1.1.179 - D-xylose 1-dehydrogenase (NADP(+)) / D-xylose-NADP dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCACGGTAGGCACGGGAGCCGCCACG |
| Internal bar code: | GAGGGTTCCTCCCTCCAGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 96.25 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGCACTCTGGTTTTCCT |
| Suggested primer 2: | GAGAGTCTTGTGAGGTCCGC |