| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256901 |
| Chromosome: | chromosome 5 |
| Location: | 2143828 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234642 | (1 of 16) PF07707 - BTB And C-terminal Kelch (BACK) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTCTTCCTGGAGCTCGGGTGTGATGGT |
| Internal bar code: | GCCGGTAGCAGCATACATGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 90.7407 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCATTCCTAGCGTCAG |
| Suggested primer 2: | TCATACAAGTCCTTGCAGCG |