| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256917 |
| Chromosome: | chromosome 1 |
| Location: | 5106542 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035500 | VPS34 | Vacuolar protein sorting 34; (1 of 1) 2.7.1.137 - Phosphatidylinositol 3-kinase / Type III phosphoinositide 3-kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACATCGACAGACGCAAGACGTGTGGGC |
| Internal bar code: | GGTTCCCATAGGACCAACCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 98.4925 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAGTTAGGACGTGCGTG |
| Suggested primer 2: | ACCGGTAGAAAGAAGGCGAT |