| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256945 |
| Chromosome: | chromosome 17 |
| Location: | 52164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696500 | PHC19 | Pherophorin-chlamydomonas homolog 19; (1 of 71) PF12499 - Pherophorin (DUF3707) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGTTTGGGCCCGTGTGGGTTTCAGGCC |
| Internal bar code: | CCGGACAAGCAGGTGCACCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 99.1936 |
| LEAP-Seq n confirming: | 369 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCATCCCACACCATCTAT |
| Suggested primer 2: | CCCAGATAGCTCCAGACAGC |