| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256955 |
| Chromosome: | chromosome 7 |
| Location: | 4243138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341900 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATGTGTGCCATTGCGTGATTGTGATGC |
| Internal bar code: | GACTGAGTTCGTATGCCCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 97.9715 |
| LEAP-Seq n confirming: | 1787 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGGTTTCGATTTTGGA |
| Suggested primer 2: | GAGGTGGAGAACGGAGTGAG |