Insertion junction: LMJ.RY0402.256964_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.256964
Chromosome:chromosome 13
Location:3732638
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGAACGCCCACCCGCCCGCGCCCGGAAC
Internal bar code:GGTATCGCGGTCCTGGCGATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:417
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGCTAACCACCTCCATCA
Suggested primer 2:CAAGCCATACGACAGCAAGA