| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256964 |
| Chromosome: | chromosome_13 |
| Location: | 3732638 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589350 | FBB6 | Flagellar/basal body protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGAACGCCCACCCGCCCGCGCCCGGAAC |
| Internal bar code: | GGTATCGCGGTCCTGGCGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTAACCACCTCCATCA |
| Suggested primer 2: | CAAGCCATACGACAGCAAGA |