| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256970 |
| Chromosome: | chromosome 13 |
| Location: | 4372820 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602950 | (1 of 1) IPR027050 - Nuclease SbcCD subunit C | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTTCTCCCCTCCTTTACGCCTTGCGAC |
| Internal bar code: | ATGTGCATGTTTTACCTGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 98.9172 |
| LEAP-Seq n confirming: | 1279 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGACCAATAGAAGCTGGC |
| Suggested primer 2: | TAGAGCCGATGCTGTGTGTC |