| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256988 |
| Chromosome: | chromosome 3 |
| Location: | 5929324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190200 | (1 of 1) IPR012351 - Four-helical cytokine, core | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGCCCCCGGGCAACATATGCAATCGAA |
| Internal bar code: | TCATTAGTCAGTTGTCGTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 98.0993 |
| LEAP-Seq n confirming: | 1858 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTTTCGTTTCGGTCTC |
| Suggested primer 2: | TCGACTTATCGCTGTTGTGC |