Insertion junction: LMJ.RY0402.256995_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256995
Chromosome:chromosome 12
Location:8835131
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGAAGTCGCTGTCCCTGAGTCCGATTCC
Internal bar code:GGGGGCACCCGGGTTTAATGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:47
LEAP-Seq percent confirming:72.973
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACAGCTGGTACCGGTAGGG
Suggested primer 2:GAGGGAGGGACTGGGATAAG